Composite

Part:BBa_K1371040:Design

Designed by: Yiran Wu   Group: iGEM14_SCUT-China   (2014-10-07)

DEBS1(KR1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1876
    Illegal BamHI site found at 6423
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 2039
    Illegal NgoMIV site found at 2411
    Illegal NgoMIV site found at 2444
    Illegal NgoMIV site found at 2917
    Illegal NgoMIV site found at 3626
    Illegal NgoMIV site found at 4499
    Illegal AgeI site found at 367
    Illegal AgeI site found at 1729
    Illegal AgeI site found at 2075
    Illegal AgeI site found at 2654
    Illegal AgeI site found at 4375
    Illegal AgeI site found at 4445
    Illegal AgeI site found at 5072
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1481
    Illegal BsaI.rc site found at 3911
    Illegal SapI.rc site found at 2746


Design Notes

Candidate selection

Biosynthesis of polyketides like erythromycin, tacrolimus and many others are guided by modular PKS genes. These genes encode large enzymes consisting of modules of domains, forming an "assembly line" to extend the ketide units to form polyketides. Among the known PKS genes, the genes coding for erythromycin producing polyketide synthethase have been extensively and deeply studied, and there are numerous information sources. Therefore, we chose a truncated erythromycin-producing polyketide synthase which has been proved to produce a simple triketide lactone 1B.[2]


Source

Saccharopolyspora erythraea

References

[1]David E. Cane, Programming of Erythromycin Biosynthesis by a Modular Polyketide Synthase, J. Biol. Chem. (2010) 285:27517-27523.

[2]Blaine A. Pfeifer, Suzanne J. Admiraal, Hugo Gramajo, David E. Cane, Chaitan Khosla. Biosythesis of Complex Polyketides in a Metabolically Engineered Strain of, E.coli [J]. Science, 2001, 291(44): 1790-1792.

[3]Tae, Hongseok,Development of an Analysis Program of Type I Polyketide Synthase Gene Clusters Using Homology Search and Profile Hidden Markov Model, J. Microbiol. Biotechnol. (2009), 19(2), 140–146.

[4]Patrick Caffrey, Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes Chemistry & Biology 8 (2001) 713-723.